兔 anti-小鸡 IgY (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgY products
- IgY
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 603-4502
- Supplier
- Rockland
- 原理
- Chicken IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, and Chicken IgY / IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
- 产品特性
- Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
- 纯化方法
- Anti-Chicken IgY / IgG Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY / IgG coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Chicken IgY / IgG whole molecule
- 亚型
- IgG
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Chicken anti-Chicken IgY antibody (DyLight 633)
IF, WB Polyclonal IgY DyLight 633
Chicken anti-Chicken IgY antibody (DyLight 405)IF, WB Polyclonal IgY DyLight 405
Chicken anti-Chicken IgY antibody (DyLight 488)IF, WB Polyclonal IgY DyLight 488
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Chicken anti-Chicken IgY antibody (DyLight 550)IF, WB Polyclonal IgY DyLight 550
Mouse anti-Chicken IgY antibody (PE)ELISA Monoclonal G-1 IgG1 PE
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (PE)ELISA Polyclonal IgG PE
Mouse anti-Chicken IgY antibodyELISA Monoclonal G-1 IgG1 unconjugated
Mouse anti-Chicken IgY antibody (Biotin)ELISA Monoclonal G-1 IgG1 Biotin
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- 应用备注
- Application Note: Anti-Chicken IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store Secondary Antibody at 4° C before opening. DO NOT FREEZE. This antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgY
- 别名
- IgY,IgG (IgY 产品)
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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