兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 603-4102
- Supplier
- Rockland
- 原理
- Chicken IgG (H&L) Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
- 产品特性
- Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Chicken IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Chicken IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Chicken IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Chicken IgG antibody (DyLight 405)WB, FLISA, FM Polyclonal IgG DyLight 405
Goat anti-Chicken IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Chicken IgG antibody (DyLight 405) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 405
Goat anti-Chicken IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
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- 应用备注
- Application Note: Anti-Chicken IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:10,000 - 1:30,000 ELISA Dilution: 1:200,000 - 1:400,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 9.973 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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