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兔 anti-Cow IgG (F(ab')2 Region) Antibody (TRITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG TRITC
产品编号 ABIN100930
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2382
    • 512
    • 423
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    F(ab')2 Region
    适用
    • 724
    • 699
    • 602
    • 477
    • 403
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    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    宿主
    • 1778
    • 1575
    • 572
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    克隆类型
    • 3434
    • 175
    • 1
    多克隆
    标记
    • 588
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    TRITC
    应用范围
    • 1953
    • 1837
    • 1807
    • 1520
    • 933
    • 909
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
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    • 53
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    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG F(ab')2
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Bovine IgG coupled to agarose beads
    免疫原

    Immunogen: Bovine IgG F(ab')2 fragment

    亚型
    IgG
    Labeling Ratio
    2.7
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Bovine IgG F(ab')2 Rhodamine Conjugated Antibody, rabbit anti-Bovine IgG F(ab')2 Rhodamine Conjugated Antibody

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

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