山羊 anti-Cow IgG (F(ab')2 Region) Antibody (TRITC) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- F(ab')2 Region
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适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- IgG F(ab')2
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Bovine IgG F(ab')2 fragment
- 亚型
- IgG
- Labeling Ratio
- 3.0
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Bovine IgG F(ab')2 fragment Rhodamine Conjugated Antibody, goat anti-Bovine IgG Fab2 fragment Rhodamine Conjugated Antibody
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
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