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山羊 anti-人 IgA, IgG, IgM Antibody (Alkaline Phosphatase (AP))

Rockland 609-105-130 ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
产品编号 ABIN100789
Supplier Product No.: 609-105-130
发货至: 中国
  • 抗原 See all IgA, IgG, IgM products
    IgA, IgG, IgM
    适用
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    宿主
    • 160
    • 55
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    山羊
    克隆类型
    • 216
    • 2
    多克隆
    标记
    • 54
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    • 10
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    Alkaline Phosphatase (AP)
    应用范围
    • 116
    • 112
    • 78
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    • 69
    • 56
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Supplier Product No.
    609-105-130
    Supplier
    Rockland
    原理
    Human IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.  This reagent is suitable for the detection of all human immunoglobulin isotypes, subclasses and chain combinations.
    产品特性
    Anti-Human IgG (H&L) Alkaline Phosphatase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    过滤
    Sterile filtered
    免疫原
    Optional[Immunogen]: Human IgG, IgA and IgM whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Anti-Human IgG IgA IgM Alkaline Phosphatase conjugate has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.1 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    有效期
    12 months
  • 抗原
    IgA, IgG, IgM
    别名
    IgG,IgA,IgM (IgA, IgG, IgM 产品)
    物质类
    Antibody
    背景
    Anti-Human IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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