山羊 anti-人 IgA Antibody (Alkaline Phosphatase (AP))
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- 抗原 See all IgA products
- IgA
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 609-1506
- Supplier
- Rockland
- 原理
- Human IgA (alpha chain) Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against Human IgG F(c). Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
- 产品特性
- Anti-Human IgG F(ab')2 Alkaline Phosphatase Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Human IgA alpha heavy chain
- 亚型
- IgG
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Rabbit anti-Human IgA antibody
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
Mouse anti-Human IgA (Heavy Chain) antibodyELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Goat anti-Human IgA antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
Goat anti-Human IgA (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
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- 应用备注
- Application Note: Anti-Human IgA Alkaline Phosphatase Antibody has been tested by dot blot and ELISA and is assayed against 1.0 μg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:7,000 is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
- 背景
- Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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