cutrun-combo
Julian PampelCUT&RUN stands for "Cleavage Under Targets and Release Using Nuclease". The technique developed from the Henikoff Lab offers a new approach to pursue epigenetics. CUT&RUN introduces some major modifications to eliminate shortcomings inherent to ChIP-seq. It is simple to perform and inherently robust, with extremely low backgrounds, while requiring only ~1/10th the sequencing depth as ChIP. As a result, less sample material is needed, making CUT&RUN a cost-effective option for transcription factor and chromatin profiling.
Assemble Your CUT&RUN Combo
antibodies-online has a special offer for our CUT&RUN customers: A customizable CUT&RUN set, the CUT&RUN Combo. The CUT&RUN Combo gives you a head start in CUT&RUN with concanavalin A beads, a wide selection of antibodies validated for CUT&RUN, control antibodies, pAG-MNase and more, all combined in one set for at least 50 reactions. Benefit from antibodies-online’s expertise - our broad offer of validated CUT&RUN antibodies satisfy the highest standards.
Do you want to perform CUT&RUN experiments but have trouble finding the right reagents? Follow the instructions below to get your customized CUT&RUN Combo for only 600 € in total. Get started now!
- Browse our list of CUT&RUN Combo components down below and select:
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- Concanavalin A beads
- Primary Antibody
- Control Antibodies
- pAG-MNase
- Select products for your personal experimental needs
- Get in touch with our team
- Get your personalized CUT&RUN Combo for 600 € *
Customize Your Combo
Pick Your CUT&RUN Components
Concanavalin A beads are responsible for cell immobilization. The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them suitable for isolation of glycan-presenting cells. For CUT&RUN Magnetic ConA Beads (Agarose) offer easier washing and resuspension steps, while reducing the risk of samples drying out. The negative control antibody is used to establish a reference background for peak calling. This is necessary because of the sparse background signal in CUT&RUN samples compared to ChIP-seq samples. On the other hand, the positive control indicates nonspecific binding of the antibody directed against the protein of interest to other proteins in the sample and is useful to avoid identification of false positive signals. The antibody of interest is determined by the target you want to investigate. For guidance, browse our tables organized by different research focuses such as histone modification or transcription regulation down below. Last, is the pAG-MNase. This enzyme construct of protein A and G is fused to micrococcal nuclease and responsible for DNA digestion prior to chromatin release.
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Positive Control
ProductSourceReactivityApplicationCat. No.ValidationsQuantityDatasheetSource Guinea PigReactivity RabbitApplication ELISA, IHC, WB, CUT&Tag, CUT&RUNCat. No. ABIN101961Validations- (105)
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Quantity 200 μLDatasheet DatasheetSource RabbitReactivity MouseApplication ELISA, IHC, WB, CUT&Tag, CUT&RUNCat. No. ABIN101785Validations- (4)
- (1)
Quantity 2 mgDatasheet DatasheetNegative Control
ProductSourceReactivityApplicationCat. No.ValidationsQuantityDatasheetSource RabbitReactivity HumanApplication WB, IF, ChIP, IP, ChIP-seq, CUT&RUNCat. No. ABIN3023251Validations- (4)
- (21)
- (1)
Quantity 100 μLDatasheet DatasheetSourceReactivityApplication CUT&RUN, CUT&TagCat. No. ABIN6923144Validations- (1)
- (1)
Quantity 135 μLDatasheet Datasheet -
Histone Modification Browse Antibodies arrow_forward
Transcription Regulator Browse Antibodies arrow_forward
Chromatin Remodeler Browse Antibodies arrow_forward
NA Metabolism Browse Antibodies arrow_forward
Histone Modification
ProductSourceClonalityApplicationCat. No.ValidationsQuantityDatasheetSource RabbitClonality PolyclonalApplication WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&TagCat. No. ABIN2668475Validations- (23)
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Quantity 100 μgDatasheet DatasheetSource MouseClonality MonoclonalApplication WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&TagCat. No. ABIN2668403Validations- (4)
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Quantity 100 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&TagCat. No. ABIN2668472Validations- (75)
- (5)
Quantity 100 μLDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&TagCat. No. ABIN3023254Validations- (5)
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Quantity 100 μLDatasheet DatasheetSource MouseClonality MonoclonalApplication IF, FACS, IHC, StM, CUT&RUNCat. No. ABIN6939594Validations- (8)
Quantity 100 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUNCat. No. ABIN3434046Validations- (5)
- (5)
Quantity 100 μLDatasheet DatasheetSource MouseClonality MonoclonalApplication WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&TagCat. No. ABIN2668392Validations- (2)
- (5)
Quantity 100 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUNCat. No. ABIN6971937Validations- (4)
Quantity 100 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, SNAP-ChIPCat. No. ABIN6952339Validations- (1)
Quantity 50 μgDatasheet DatasheetSource RabbitClonality MonoclonalApplication WB, ELISA, ChIP, ICC, MA, CUT&RUNCat. No. ABIN2830942ValidationsQuantity 100 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeACat. No. ABIN6952337ValidationsQuantity 50 μgDatasheet DatasheetTranscription Regulator
ProductSourceClonalityApplicationCat. No.ValidationsQuantityDatasheetSource RabbitClonality PolyclonalApplication WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUNCat. No. ABIN2855074Validations- (11)
- (15)
- (1)
Quantity 100 μLDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IHC, IF, FACS, IHC (p), IP, ICC, IHC (fro), ChIP, IHC (wm), CUT&RUNCat. No. ABIN2855042Validations- (8)
- (14)
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Quantity 100 μLDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, ELISA, ICC, IF, CUT&RUNCat. No. ABIN6265491Validations- (3)
- (1)
Quantity 100 μLDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ICC, CUT&RUN, ChIP-seq, ChIPCat. No. ABIN6972778Validations- (1)
- (3)
Quantity 100 μLDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ICC, ChIP-seq, ChIP, CUT&RUNCat. No. ABIN6972403Validations- (3)
Quantity 100 μLDatasheet DatasheetChromatin Remodeler
ProductSourceClonalityApplicationCat. No.ValidationsQuantityDatasheetSource RabbitClonality PolyclonalApplication WB, ChIP-seq, ChIP, CUT&RUNCat. No. ABIN6971459Validations- (3)
Quantity 100 μLDatasheet DatasheetNA Metabolism
ProductSourceClonalityApplicationCat. No.ValidationsQuantityDatasheetSource MouseClonality MonoclonalApplication WB, ELISA, ChIP, FM, CUT&RUNCat. No. ABIN6655367Validations- (7)
Quantity 50 μgDatasheet DatasheetSource MouseClonality MonoclonalApplication WB, ELISA, ChIP, FM, CUT&RUNCat. No. ABIN6655366Validations- (7)
Quantity 50 μgDatasheet DatasheetSource RabbitClonality PolyclonalApplication WB, IF, ICC, IHC (p), CUT&RUNCat. No. ABIN2856044Validations- (3)
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Quantity 100 μLDatasheet Datasheet -
ProductApplicationCat. No.ValidationsQuantityDatasheetApplication ChIC, CUT&RUNCat. No. ABIN6950951Validations
- (4)
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Quantity 50 reactionsDatasheet Datasheet
antibodies-online: Paving the Way for CUT&RUN
The successful execution of CUT&RUN hinges on antibody selection. In order to cut adjacent DNA, the pA/G-MNase fusion protein needs to recognize the targeted chromatin part. As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and specificity under the conditions used for binding.
We Help Scientists Find the Right Antibody
Benefit from antibodies-online years of expertise. Since the development of the CUT&RUN technique at the Henikoff Lab we aim to improve the technique. We share our experience with our customers and guide them to find the right antibody.
antibodies-online is the leading vendor-independent marketplace for research reagents, especially antibodies and ELISA kits. The company was founded in 2006 as a spin-off from RWTH Aachen University (Germany). We deliver research products to customers in over 70 different countries. We currently list over 1,95 million research products, which we deliver to universities, biotech and pharmaceutical companies worldwide.
Our diverse team consists of highly motivated biologists, IT- and business specialists. Together we strive to provide scientists with the best possible support for their research.
