AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KA
产品特性
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human neutrophils is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rMuMIP-2/CXCL2 as determined by LAL method
CXCL2
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Macrophage Inflammatory Protein 2 (MIP-2) was originally identified as a heparin binding protein secreted from a mouse macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (CXC) subfamily of chemokines.Similarly to other alpha chemokines, mouse MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the mouse interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases. Synonym: MIP-2/CXCL2, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
分子量
7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.