Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.
纯度
> 96 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuMCP-4/CCL13 as determined by LAL method
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13. Synonym: MCP-4/CCL13, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.
分子量
8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids.