Fully biologically active when compared to standard. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED50 for this effect is typically 0.03-0.1µg/mL.
纯度
> 96 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuMCP-2/CCL8 as determined by LAL method
CCL8
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % by SDS-PAGE analysis.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1.MCP-3 also shares 58% amino acid identity with MCP-2.Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition,the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils. Synonym:. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.
分子量
8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.