Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuMDC/CCL22 as determined by LAL method
CCL22
宿主: 小鼠
宿主: CHO Cells
Recombinant
>98 % (SDS-PAGE)
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoattracts monocytes, dendritic cells and NK cells and exerts HIV suppressive activity. Synonym: Macrophage-Derived Chemokine/CCL22 (69a.a.), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH7.4, 500mM NaCl.
分子量
8.1 kDa, a single, non-glycosylated polypeptide chain containing 69 amino acids.