It is recommended to reconstitute the lyophilized rHuIL-8 /72 in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Specificity rHuIL-8 /72 is fully biologically active when compared to standard. The ED50 as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells is less then 0.5 ng/ml, corresponding to a specific activity of 2.0×106 IU/mg.
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states. GenScript Interleukin (IL)-8 (8-79), produced in E. coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8,452 Da. Synonym: Interleukin (IL)-8 (8-79). Formulation: The protein was lyophilized after extensive dialysis against PBS, pH7.4.