IL8
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95.0 %, as determined by the following methods: (a) RP-HPLC analysis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
It is recommended that lyophilized Recombinant Human Interleukin-8 (IL-8)/77 be reconstituted in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Specificity Recombinant Human Interleukin-8 (IL-8)/77 is fully biologically active when compared to standard. The ED50, as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells, is less than 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/mg.
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 (IL-8) levels are associated with the onset of a variety of disease states. Human Interleukin-8 (IL-8) (3-79) , produced in E. coli, is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8,904 Da. Synonym: rHu IL-8 (3-79). Formulation: The protein was lyophilized after extensive dialysis against PBS, pH7.4.