Fully biologically active when compared to standard.Measured by its ability to induce STAT3 activation in U-87 MG human glioblastoma/astrocytoma cells. 5 ng/mL of rhIL31 can effectively induce STAT3 activation.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuIL-31 as determined by LAL method
IL31
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
Human IL-31 is a T-cell derived cytokine that shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. It signals through a receptor complex comprised of GPL (GP130-like, IL-31RA) and OSMR (Oncostatin M receptor). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Synonym:. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS,pH 7.4.
分子量
Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.