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IFN alpha 2b 蛋白

宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 95.0 % as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Active
产品编号 ABIN987980
发货至: 中国
  • 抗原 See all IFN alpha 2b products
    IFN alpha 2b
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
    资源
    • 2
    • 1
    • 1
    大肠杆菌(E. Coli)
    序列
    analysis The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro. N-terminal methionine has been completely removed enzymatica lly. p to grams level.
    产品特性
    The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1.0×108IU/mg.
    纯度
    > 95.0 % as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
    内毒素水平
    Level Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Interferon alpha 2b (IFN alpha2b
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is recommended to reconstitute the lyophilized Recombinant Human Interferon alpha 2b (IFN alpha2b) in sterile 18M-omega-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard.
    储存条件
    -20 °C
  • Perales, Beach, Gallego, Soria, Quer, Esteban, Rice, Domingo, Sheldon: "Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype." in: Journal of virology, Vol. 87, Issue 13, pp. 7593-607, (2013) (PubMed).

  • 抗原
    IFN alpha 2b
    别名
    IFN-alpha 2b (IFN alpha 2b 产品)
    别名
    IFN-alphaA Protein, IFNA Protein, IFNA2B Protein, INFA2 Protein, interferon alpha 2 Protein, IFNA2 Protein
    背景
    Human Interferon alpha 2b (IFN alpha2b) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon alpha 2b (IFN alpha2b) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2b (IFN alpha2b) is purified by proprietary chromatographic techniques. Synonym: rHuIFN-alpha-2b, rHuIFNalpha-2b, rHuIFN-a-2b, rHuIFN-alfa-2b, rHuIFNalfa-2b, Interferon-alpha-2b, Interferonalpha-2b, Interferon-a-2b, Interferon-alfa-2b, Interferonalfa-2. Formulation: Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.
    分子量
    19.4kDa +/- 10% Isoelectric Point The main zone between 5.5-6.8 analysis by IEF. UV Scan The maximal absorption wave is 278+/-3 nm.
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