Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 75 ng/ml, corresponding to a specific activity of >, 1.3 × 104 IU/mg.
纯度
> 96 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuFGF-7/KGF-1 as determined by LAL method
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Keratinocyte Growth Factor-1 (KGF-1/FGF-7) is one of 23 known members of the FGF family. All FGFs have two conserved cysteine residues and share 30 - 50% sequence identity at the amino acid level. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF-1/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF-1/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Synonym: Fibroblast Growth Factor-7 ( FGF-7), Human. Formulation: Lyophilized from a 0.2µm filtered solution in 20mM PB, pH 8.0, 1M NaCl.
分子量
Approximately 19.0 kDa, a single, non-glycosylated polypeptide chain containing 164 amino acids.