EPO Protein (AA 28-193)
EPO
宿主: 人
宿主: CHO Cells
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
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- 抗原 See all EPO 蛋白
- EPO (Erythropoietin (EPO))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-193
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宿主
- 人
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资源
- CHO Cells
- 产品特性
- ED50< 1 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >1 x 10^6 units/mg
- 纯度
- > 95 % as analyzed by SDS-PAGE and HPLC.
- 内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Erythropoietin (EPO) (AA 1-193) protein (GST tag)
EPO 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin (EPO) (Active) protein (His tag)EPO 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
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- 应用备注
- Mature human EPO, containing 166 amino acid residues, has a predicted molecular mass of approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26-36 kDa in SDS-PAGE.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against PBS.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant human EPO remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhEPO should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
- 有效期
- 6 months
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- 抗原
- EPO (Erythropoietin (EPO))
- Abstract
- EPO 产品
- 别名
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- 物质类
- Hormone
- 背景
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Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.The cDNAs for EPO have been cloned from human, mouse, canine, etc. The mature proteins from the various species are highly conserved, exhibiting greater than 80 % sequence identity at the amino acid level. Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein. EPO contains one O-linked and three N-linked glycosylation sites. Glycosylation of EPO is required for EPO biological activities in vivo. EPO exhibits structural as well as amino sequence identity to the amino terminal 153 amino acid region of thrombopoietin.
Synonyms: Human EPO-alpha, EPO-alpha, EPO alpha, EPOalpha, h-EPO-alpha, rh-AEPO-alpha, recombinant human EPO-alpha, recombinant EPO-alpha, EPO. - 分子量
- 21 kDa, observed 26-36 kDa in SDS-PAGE.
- 途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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