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EGF 蛋白

Name: EGF 蛋白
Brand: antibodies-online
SKU: ABIN987821
Availability: OutOfStock

EGF 宿主: 人宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active

2 μg of EGF, Human was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining. (EGF 蛋白)

SDS

Image no. 1 for Epidermal Growth Factor (EGF) (Active) protein (ABIN987821) (EGF 蛋白)

EGF, Human stimulates cell proliferation of the Balb/3T3 Cells. (EGF 蛋白)

AcA

3 images

(1 reference)

产品编号 ABIN987821

发货至: 中国

Datasheet as PDF

抗原 See all EGF 蛋白

EGF (Epidermal Growth Factor (EGF))

生物活性

Active
宿主
20

13

9

4

3

1
人
资源
31

9

2

2

1

1

1

1
大肠杆菌（E. Coli）

序列

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW EL

产品特性

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg.

纯度

> 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

内毒素水平

Level Less than 0.1ng/myg (1 IEU/myg) determined by LAL test

Top Product

Discover our top product EGF 蛋白
Epidermal Growth Factor (EGF) protein
EGF 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)
EGF 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)
EGF 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)
EGF 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Epidermal Growth Factor (EGF) (Active) protein
EGF 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active

Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)
EGF 宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)
EGF 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Epidermal Growth Factor (EGF) protein
EGF 宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant

Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (Fc Tag)
EGF 宿主: 小鼠宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Epidermal Growth Factor (EGF) (AA 977-1023) protein (GST tag)
EGF 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA

限制

仅限研究用
状态

Lyophilized

溶解方式

It is recommended to reconstitute the lyophilized Recombinant Human Epidermal Growth Factor (EGF) in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.

储存条件

-20 °C
Li, Niu, Lang, Guo, Ma, Kiesewetter, Backer, Shen, Chen: "PET imaging of EGF receptors using [18F]FBEM-EGF in a head and neck squamous cell carcinoma model." in: European journal of nuclear medicine and molecular imaging, Vol. 39, Issue 2, pp. 300-8, (2012) (PubMed).
抗原

EGF (Epidermal Growth Factor (EGF))

Abstract

EGF 产品

别名

HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein

背景

Human Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. Synonym: rHuEGF, Epidermal Growth Facto. Formulation: The protein was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.

分子量

6.0 kDa+/-10% determined by reduced SDS-PAGE Isoelectric Point The main zone between 4.0-5.0 analysis by IEF UV Scan The maximal absorption wave is 275+/-3 nm.

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation