Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human peripheral blood eosinophils is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuEotaxin-2/CCL24 as determined by LAL method
CCL24
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Eotaxin, also named MPIF-2 and Ckbeta6, is a novel CC chemokine recently identified. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 selectively chemoattracts cells expressing CCR3 including eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells. Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 a.a. residues for the mouse homolog, without C-terminal truncation). Synonym: Eotaxin-2/CCL24, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
分子量
8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.