The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0 x 107units/mg.
纯度
> 98 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuBetacellulin as determined by LAL method
BTC
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % by SDS-PAGE analysis.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
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背景
Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Synonym: Betacellulin ( BTC), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
分子量
Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.