Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay usingimmature human dendritic cells is less than 100 ng/ml, corresponding to a specific activity of >, 1.0 × 104 IU/mg.
纯度
> 98 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuBD-2 as determined by LAL method
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Synonym: beta-Defensin 2 (BD-2), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.
分子量
Approximately 4.3 KDa, a single non-glycosylated polypeptide chain containing 41 amino acids.