Transferrin Protein (TF)
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- 抗原 See all Transferrin (TF) 蛋白
- Transferrin (TF)
- 蛋白类型
- Native
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宿主
- 人
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资源
- Human
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Supplier Product No.
- 009-0134
- Supplier
- Rockland
- 原理
- Human Transferrin- 009-0134
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.
- 产品特性
- apotransferrin, Transferrin Control, Siderophilin, TRF, apo-hTF
- 纯化方法
- Human transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis.
- Top Product
- Discover our top product TF 蛋白
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- 应用备注
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Application Note: Human Apo Transferrin from human serum was lyophilized from 0.2M ammonium bicarbonate for use in cell culture and assays. Transferrin has been tested in SDS-Page and is also suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified proteins.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:10,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: See application note.
Stabilizer: None
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Transferrin (TF)
- 别名
- Transferrin- (TF 产品)
- 背景
- Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality.
- 基因ID
- 7018
- UniProt
- Q06AH7
- 途径
- Transition Metal Ion Homeostasis
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