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Protein A Protein (Alkaline Phosphatase (AP))

宿主: Staphylococcus aureus Recombinant ELISA
产品编号 ABIN964522
发货至: 中国
  • 抗原 See all Protein A 蛋白
    Protein A
    蛋白类型
    Recombinant
    宿主
    Staphylococcus aureus
    宿主
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    标记
    This Protein A protein is labelled with Alkaline Phosphatase (AP).
    应用范围
    ELISA
    特异性
    Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
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  • 应用备注
    Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
    说明

    Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液
    0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    Protein A
    Abstract
    Protein A 产品
    别名
    alpha protein Protein, repB Protein
    背景
    Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
    Synonyms: ProA, ALP, Alk Phos
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