Purified recombinant Human PAI1 protein Expression System: E.coli Bioactivity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 °C. Half maximal inhibition against 1.0 µg/mL of single chain tPA was obtained at a concentration of 2.0 µg/mL.
Plasminogen Activator Inhibitor-1 (PAI-1, Serpin E1) is a member of the serpin family of serine protease inhibitors, and is the primary inhibitor of urokinase and tissue plasminogen activator (tPA). PAI-1 is expressed predominantly in adipose, liver and vascular tissues, and is also produced by certain tumor cells. Elevated levels of PAI-1 are associated with obesity, diabetes and cardiovascular disease, and increased production of PAI-1 is induced by various obesity related factors such as TNFa, glucose, insulin, and very-low-density lipoprotein. The obesity related elevation of PAI-1 levels along with the consequential deficiency in plasminogen activators can lead directly to increased risk of thrombosis and other coronary diseases. Alternative Names: PAI-1, PAI 1, PAI-1 protein, PAI 1 protein, PAI1, PAI-1 protein, Serpin E1 protein, Plasminogen Activator Inhibitor-1 protein