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FKBP3 Protein (AA 1-224)

FKBP3 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 90 % pure SDS Active
产品编号 ABIN935093
发货至: 中国
  • 抗原 See all FKBP3 蛋白
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 1-224
    宿主
    • 8
    • 2
    • 2
    • 1
    • 1
    资源
    • 9
    • 3
    • 1
    • 1
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS)
    序列
    MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID
    产品特性
    Purified recombinant Human FKBP3 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 120 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
    纯度
    > 90 % pure
    Top Product
    Discover our top product FKBP3 蛋白
  • 应用备注
    FKBP3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    实验流程
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant FKBP3 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
    储存液
    Dithiothreitol (DTT)
    注意事项
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze/thaw cycles.
    储存条件
    RT/-20 °C
    储存方法
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • 抗原
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    别名
    FKBP3 (FKBP3 产品)
    别名
    FKBP3 Protein, 25kDa Protein, FKBP-3 Protein, FKBP25 Protein, FK506 Protein, fb75c04 Protein, si:dz261o22.2 Protein, wu:fb75c04 Protein, zgc:110728 Protein, FKBP-25 Protein, PPIase Protein, FK506 binding protein 3 Protein, FK506 binding protein 3 L homeolog Protein, peptidylprolyl isomerase Protein, FKBP3 Protein, Fkbp3 Protein, fkbp3 Protein, fkbp3.L Protein, CAALFM_C103790CA Protein
    背景
    FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: PPIase protein, FK506 binding protein 3 FKBP 25 protein, FKBP-3 protein, FKBP3, PPIase FKBP3 protein, FKBP-3, Rotamase protein, Rotamase., FKBP25 protein, FKBP 3, FKBP 3 protein, FKBP 3 protein
    分子量
    25.1 kDa (224 AA)
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