GSTA1 Protein (AA 1-222)
GSTA1
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % pure
SDS
Active
1 image
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- 抗原 See all GSTA1 蛋白
- GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-222
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
- 产品特性
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Purified recombinant Human GSTA1 protein
Expression System: E.coli
Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C. - 纯度
- > 90 % pure
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- Discover our top product GSTA1 蛋白
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Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) protein
GSTA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) protein (GST tag)GSTA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
3.2 % (v/v) ethanol.
2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
3. Add 50 µL of recombinant GSTA1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
4. Mix by inversion and record the increase at A340 nm for 5 minutes.
- Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))
- 别名
- GSTA1 (GSTA1 产品)
- 背景
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GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GSTA-1 protein, GSTA 1 protein, HA subunit 1., GTH1 protein, Glutathione S-transferase alpha 1 Glutathione S transferase A1 protein, GSTA1 1 protein, GSTA1, GSTA-1, GST epsilon protein, GSTA1-1 protein, GST2 protein, GSTA 1, GSTA1 protein - 分子量
- 25.6 kDa (222 AA)
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