SlyD (AA 1-196) (Active) 蛋白
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % pure
SDS
Active
1 image
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- 抗原
- SlyD
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-196
- 宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
- 产品特性
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Purified recombinant E.coli SlyD protein
Expression System: E.coli
Bioactivity: Specific activity is > 66 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
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SlyD (AA 1-196) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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- 应用备注
- SlyD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
2. Add recombinant SlyD protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
3. Mix by inversion and equilibrate to 1C and monitor the A410 nm until the value is constant using a spectrophotometer.
4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
5. Record the increase in A410 nm for 20 minutes
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 20 mM Tris, pH 7.5.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- SlyD
- 背景
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SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. coli slyD was expressed in E. coli and purified by conventional chromatography.
Alternative Names: WHP., FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD protein, Histidine rich protein protein, FKBP-type peptidyl-prolyl cis-trans isomerase protein, Rotamase protein, PPIase protein, SlyD protein - 分子量
- 21 kDa (196 AA)
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