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- 抗原
- DnaK
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-384, N-Term
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宿主
- 所有种类
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG
- 产品特性
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Purified recombinant E.coli Dnak protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
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DnaK (AA 1-638), (full length) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 385-546) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 508-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 385-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-384) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-638), (full length) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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- 应用备注
- Dnak protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in 25 mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5 mM DTT, and 10 % glycerol.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- DnaK
- 背景
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DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
Alternative Names: dnaK protein, Heat shock 70 kDa protein protein, 1-384) protein, Co chaperone with DnaJ protein, Chaperone protein dnaK protein, HSP70 protein, Heat shock protein 70 protein, Dnak (N-term protein, ATPase binding domain protein, Chaperone Hsp70 protein, - 分子量
- 41.6 kDa (384 AA)
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