HIV-2 gp36 protein (His tag)
宿主: 人
宿主: Yeast (Pichia pastoris)
Recombinant
ELISA, WB
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- 抗原
- HIV-2 gp36
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- Yeast (Pichia pastoris)
- 标记
- His tag
- 应用范围
- ELISA, Western Blotting (WB)
- 产品特性
- Human Immunodeficiency Virus Type 2 (HIV-2) gp36, Recombinant ecto-domain of HIV-2 gp36. Contains a 6 histidine fusion partner. Reacts with HIV-2 positive human sera and monoclonal antibodies as determined by ELISA and Western blot. Major immunospecific band at 14 kDa, minor bands at 5, 16, 18, 28 and 42 kDa.
- 纯化方法
- Purity determined by SDS-PAGE and Western blot
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HIV-2 gp36 protein (His tag,MBP tag)
宿主: Human Immunodeficiency Virus (HIV) 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by SDS-PAGE
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- 应用备注
- Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. HIV-2 gp36 ELISA with HIV-2 positive sera at 1:2.000 with 1-100 nanograms per well gp36. Background with BSA or normal sera OD405 <0.1. Alkaline phosphatase substrate for 45 min.
- 说明
-
Infectious Disease Antigens and Toxins
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 6 M Urea, 0.02 M Tris-HCl, 0.5 M Sodium chloride, pH 7.0 to 8.0 at room temperature
- 储存液
- Without preservative
- 注意事项
- Aliquot to avoid multiple freeze thaws.
- 储存条件
- -80 °C
- 储存方法
- Store at -70 °C or below.
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- 抗原
- HIV-2 gp36
- 物质类
- Viral Protein
- 分子量
- 14 kDa
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