Flagellin Protein (FliC) (AA 1-495, active-Mutant)
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- 抗原 See all Flagellin (FliC) 蛋白
- Flagellin (FliC)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-495, active-Mutant
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宿主
- Salmonella typhimurium
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资源
- 大肠杆菌（E. Coli）
- 原理
- Flagellin (rec.) (untagged) (highly active)
- 交叉反应
- 人, 小鼠
- 产品特性
- Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.001EU/μg purified protein (LAL test, Lonza).
- Biological Activity Comment
- Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse.
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC 宿主: Borrelia burgdorferi 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, WB
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 1 mg/mL after reconstitution.
- 浓度
- 1 mg/mL
- 缓冲液
- Contains PBS.
- 注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
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- 抗原
- Flagellin (FliC)
- 别名
- Flagellin (FliC 产品)
- 背景
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FliC
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
- 分子量
- ~54kDa (SDS-PAGE)
- UniProt
- P06179
- 途径
- Inflammasome
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