BMP2
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
应用备注
Measured by the ability of BMP-2 to induce alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 μg/mL.
限制
仅限研究用
状态
Lyophilized
溶解方式
The lyophilized BMP-2 is best soluble in 50 mM acetic acid at a concentration of 0.1 mg/mL but should be also soluble in most aqueous buffers when the pH is below 6.0.
缓冲液
50 mM acetic acid
储存条件
-20 °C,-80 °C
储存方法
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted BMP-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
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背景
Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6,Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. The amino acid sequence of recombinant human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector. BMP-2 is a heparin binding protein.