MPXV A30L (AA 22-146) protein (His tag)
宿主: Mpox Virus
宿主: HEK-293 Cells
Recombinant
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
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- 抗原
- MPXV A30L
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-146
- 宿主
- Mpox Virus
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资源
- HEK-293 Cells
- 标记
- His tag
- 原理
- Recombinant Mpox (formerly known as monkeypox) virus (MPXV) envelope protein A28 homolog with C-terminal His-tag, derived from the transfected human HEK293 cells.
- 序列
- Gln22-Leu146
- 纯化方法
- His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
- 纯度
- SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
- 内毒素水平
- <0.5 EU per μg of the protein as determined by the LAL method
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
- 缓冲液
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Lyophilized from a 0.2 μm filtered solution in PBS ( pH 7.4) with 1 % mannitol and 5 % trehalose
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- MPXV A30L
- 分子量
- Recombinant protein product has a calculated molecular mass of ~14 kDa. Due to the abundant glycosylation, it migrates as approximately ~20-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
- UniProt
- Q8V4U9
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