HCAR2 蛋白
HCAR2
宿主: 人
宿主: Mammalian Cells
Synthetic Nanodisc
-
- 抗原 See all HCAR2 products
- HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))
- 蛋白类型
- Synthetic Nanodisc
-
宿主
- 人
-
资源
- Mammalian Cells
- 原理
- Human HCAR2 full length protein-synthetic nanodisc
- 产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
-
Hydroxycarboxylic Acid Receptor 2 (HCAR2) protein (Myc-DYKDDDDK Tag)
HCAR2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 说明
-
Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 有效期
- 12 months
-
- 抗原
- HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))
- 别名
- HCAR2 (HCAR2 产品)
- 别名
- GPR109A Protein, HCA2 Protein, HM74a Protein, HM74b Protein, NIACR1 Protein, PUMAG Protein, Puma-g Protein, Gpr109a Protein, Gpr109b Protein, HM74 Protein, Hcar2 Protein, PUMA-G Protein, Pumag Protein, mHM74b Protein, HCAR2 Protein, HCAR3 Protein, hydroxycarboxylic acid receptor 2 Protein, HCAR2 Protein, Hcar2 Protein, LOC527744 Protein
- 背景
- Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function]
- 分子量
- The human full length HCAR2 protein has a MW of 41.9kDa
- UniProt
- Q8TDS4
-
You are here:
