CHRM3 蛋白
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- 抗原 See all CHRM3 蛋白
- CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))
- 蛋白类型
- Synthetic Nanodisc
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宿主
- 人
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资源
- Mammalian Cells
- 原理
- Human ACM3 full length protein-synthetic nanodisc
- 产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
- Top Product
- Discover our top product CHRM3 蛋白
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Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 253-491) protein (Strep Tag,His tag)
Crystallography grade CHRM3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 1-67) protein (GST tag)CHRM3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Cholinergic Receptor, Muscarinic 3 (CHRM3) (AA 253-492) protein (His tag)CHRM3 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 有效期
- 12 months
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- 抗原
- CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))
- 别名
- ACM3 (CHRM3 产品)
- 别名
- EGBRS Protein, HM3 Protein, MACHR Protein, Chrm-3 Protein, M3 Protein, M3R Protein, cholinergic receptor muscarinic 3 Protein, cholinergic receptor, muscarinic 3 Protein, cholinergic receptor, muscarinic 3, cardiac Protein, CHRM3 Protein, Chrm3 Protein
- 背景
- The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]
- 分子量
- The human full length ACM3 protein has a MW of 66.1kDa
- UniProt
- P20309
- 途径
- Synaptic Membrane
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