FCGR2B Protein (AA 46-217) (His-Avi Tag)
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- 抗原 See all FCGR2B 蛋白
- FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 46-217
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FCGR2B protein is labelled with His-Avi Tag.
- 应用范围
- SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)
- 原理
- Human Fc gamma RIIb / CD32b protein
- 序列
- APPKAVLKLE PQWINVLQED SVTLTCRGTH SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIVLRCHSW KDKPLVKVTF FQNGKSKKFS RSDPNFSIPQ ANHSHSGDYH CTGNIGYTLY SSKPVTITVQ APGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H
- 特异性
- IgG
- 产品特性
- The sequence of the extracellular domain of human CD32b (Ala 46-Pro 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
- 纯化方法
- Nickel and SEC
- 纯度
- > 95 % by SEC-HPLC
- 内毒素水平
- <1.0 EU per mg
- Biological Activity Comment
- Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIb / CD32b protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 15 μM.
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- Discover our top product FCGR2B 蛋白
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Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-217) protein (His-Avi Tag)
FCGR2B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 40-217) protein (His tag)FCGR2B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-224) protein (His tag)FCGR2B 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) protein (His-Avi Tag,Biotin)FCGR2B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-217) protein (His-Avi Tag,Biotin)FCGR2B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 45-154) protein (GST tag)FCGR2B 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-217) protein (His tag)FCGR2B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (His tag)FCGR2B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (His tag)FCGR2B 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (Biotin,His-Avi Tag)FCGR2B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C,-80 °C
- 储存方法
- Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
- 有效期
- 12 months
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- 抗原
- FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
- 别名
- CD32b (FCGR2B 产品)
- 别名
- CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein
- 背景
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CD32B, FCGR2B, FCGRIIB, FCR2B, FCRIIB, IGFR2B, IGFRIIB
Background: Low affinity immunoglobulin gamma Fc receptor IIb, also known as FcγRIIb or CD32b, is a type I integral membrane glycoprotein. CD32b is a member of the immunoglobulin superfamily and is expressed on B cells, subsets of monocytes, macrophages and granulocytes, platelets and mast cells. CD32b binds monomeric IgG with low affinity but is efficient at binding immune complexes and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation. CD32b is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail containing the immunoreceptor tyrosine-based inhibition (ITIM) motif. The product provided only contains the extracellular portion of CD32. - 分子量
- 23.8 kDa
- UniProt
- P31994
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling
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