Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein,the EC50 of Leptin R/CD295 is 261.45 ng/mL.
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
缓冲液
Lyophilized from a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl,1 mM DTT, pH 6.5.
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,-80 °C
储存方法
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.