IgG1 Protein (His tag)
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- 抗原 See all IgG1 蛋白
- IgG1
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This IgG1 protein is labelled with His tag.
- 原理
- Active Recombinant Human IgG1 Protein
- 序列
- PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
- 特异性
- Pro100-Lys330
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <0.1EU/μg
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human Fc-gamma RII-a(CD32a) at 1 μg/mL (100 μL/well) can bind IgG1 Fc with a linear range of 0.156-3.47 μg/mL.
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IgG1 (Fab Region) protein
宿主: 人 宿主: Human Native >95 %
IgG1 (Fc Region) protein宿主: 人 宿主: Human Native >95 %
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- IgG1
- Abstract
- IgG1 产品
- 别名
- IgG1 Protein, Igh-4 Protein, VH7183 Protein, immunoglobulin heavy constant gamma 1 (G1m marker) Protein, Ighg1 Protein
- 物质类
- Antibody
- 背景
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Description: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75 % of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Name: Human IgG,IGHG1,COB1,YAP,YAP2,YAP65,YKI,YAP1,human IgG (Fc)
- 基因ID
- 3500
- UniProt
- P01857
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