Recombinant protein rMal d 1 is expressed in Escherichia coli. DNA sequence encoding 180 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,9 kDa.
纯化方法
Purified by sequential steps of ion exchange and affinity chromatography.
LOC100812065
宿主: Apple
宿主: 酵母菌
Recombinant
> 90 %
ELISA
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01 % Tween 20, pH 8.0).
储存液
Azide free
储存条件
4 °C
储存方法
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.