ELISA, Western Blotting (WB), Flow Cytometry (FACS)
原理
rBet v 7 (Betula verrucosa 7.0101)
产品特性
Recombinant protein rBet v 7 is expressed in Escherichia coli. DNA sequence encoding 186 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,7 kDa.
纯化方法
Purified by sequential steps of ion exchange and affinity chromatography.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01 % Tween 20, pH 8.0).
储存液
Azide free
储存条件
4 °C
储存方法
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.