MICB Protein (AA 1-298) (His tag)
-
- 抗原 See all MICB 蛋白
- MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-298
- 宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This MICB protein is labelled with His tag.
- 应用范围
- ELISA
- 序列
- Met 1-Gly 298
- 产品特性
- A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- 内毒素水平
- <1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Immobilized human MICB-His at 10 ug/ml (100 ul/well) can bind human NKG2D, The EC50 of human NKG2D is 0.52-1. 2 ug/ml.
- Top Product
- Discover our top product MICB 蛋白
-
MHC Class I Polypeptide-Related Sequence B (MICB) (AA 23-298) protein (His-Avi Tag)
MICB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
MHC Class I Polypeptide-Related Sequence B (MICB) protein (His-Avi Tag,Biotin)MICB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
MHC Class I Polypeptide-Related Sequence B (MICB) (AA 1-340) protein (GST tag)MICB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
MHC Class I Polypeptide-Related Sequence B (MICB) (AA 121-220) protein (GST tag)MICB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
MHC Class I Polypeptide-Related Sequence B (MICB) (AA 23-309) protein (His tag)MICB 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
-
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
-
- 抗原
- MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
- 别名
- MICB (MICB 产品)
- 别名
- PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein
- 背景
-
Abbreviation: MICB
Target Synonym: PERB11.2
Background: MHC class I polypeptide-related sequence B; also known as MICB; is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA; a closely related protein; both of which contain three extracellular immunoglobulin-like domains; but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen; binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells; CD8+αβ T cells; and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells; but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance; viral infections; and autoimmune diseases.
- 分子量
-
Calculated MW: 33 kDa
Observed MW: 45-50 kDa
- UniProt
- Q29980
- 途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
-
