Osteopontin Protein (AA 17-303) (His tag)
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- 抗原 See all Osteopontin (SPP1) 蛋白
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-303
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宿主
- Pig
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Osteopontin protein is labelled with His tag.
- 序列
- Leu 17-Asn 303
- 产品特性
- A DNA sequence encoding the Porcine OPN/Osteopontin protein (P14287) (Leu 17-Asn 303) was expressed with N-His tag.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product SPP1 蛋白
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 17-294) protein (His tag)SPP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 148-257) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 21-129) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) proteinSPP1 宿主: 人 Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 宿主: 大鼠 宿主: HEK-293 Cells > 95 % AcA, CC Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
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- 抗原
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- OPN (SPP1 产品)
- 别名
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- 背景
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Abbreviation: Porcine OPN,Osteopontin
Target Synonym: BNSP,Bone sialoprotein 1,BSP I,ETA 1,Nephropontin,Secreted phosphoprotein 1,SPP 1Urinary stone protein,Uroponti
Background: Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80 % amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage.
- 分子量
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Calculated MW: 31.46 kDa
Observed MW: 24 kDa
- UniProt
- P14287
- 途径
- Regulation of Cell Size
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