UBC Protein (AA 1-152) (His tag)
-
- 抗原 See all UBC 蛋白
- UBC (Ubiquitin C (UBC))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-152
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This UBC protein is labelled with His tag.
- 序列
- Met 1-Gly 152
- 产品特性
- A DNA sequence encoding the Human ubc/Polyubiquitin-C protein (P0CG48) (Met 1-Gly 152) was expressed with N-His tag.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product UBC 蛋白
-
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
-
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
-
- 抗原
- UBC (Ubiquitin C (UBC))
- 别名
- ubc (UBC 产品)
- 别名
- HMG20 Protein, 2700054O04Rik Protein, AI194771 Protein, Rps27a Protein, TI-225 Protein, Uba52 Protein, Ubb Protein, ubiquitin C Protein, UBC Protein, Ubc Protein
- 背景
-
Abbreviation: ubc,Polyubiquitin-C
Target Synonym: HMG20,Ubiquitin
Background: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in proteotoxic stress response and cell cycle, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
- 分子量
-
Calculated MW: 16.61 kDa
Observed MW: 16 kDa
- UniProt
- P0CG48
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation, Ubiquitin Proteasome Pathway
-
