EPO Protein (AA 28-193) (His tag)
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- 抗原 See all EPO 蛋白
- EPO (Erythropoietin (EPO))
- 蛋白类型
- Recombinant
- 产品特性
- AA 28-193
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This EPO protein is labelled with His tag.
- 序列
- Ala 28-Arg 193
- 产品特性
- A DNA sequence encoding the Human EPO protein (P01588) (Ala 28-Arg 193) was expressed with a C-His tag.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product EPO 蛋白
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Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin (EPO) (Active) protein (His tag)EPO 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
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- 抗原
- EPO (Erythropoietin (EPO))
- 别名
- EPO (EPO 产品)
- 别名
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- 物质类
- Hormone
- 背景
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Abbreviation: EPO
Target Synonym: Epoetin,EPO,Erythropoietin
Background: Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of the EPO protein is required for biological activities in vivo. The mature human EPO protein shares 75 % - 84 % amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
- 分子量
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Calculated MW: 18.15 kDa
Observed MW: 20 kDa
- UniProt
- P01588
- 途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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