RAC1 Protein (AA 1-189) (His tag)
Quick Overview for RAC1 Protein (AA 1-189) (His tag) (ABIN7505002)
抗原
蛋白类型
宿主
资源
纯度
-
-
产品特性
- AA 1-189
-
标记
- This RAC1 protein is labelled with His tag.
-
序列
- Met1-Cys189
-
产品特性
- A DNA sequence encoding theHuman Rac1 protein (P63000) (Met1-Cys189) was expressed with a N-His.
-
-
RAC1 (AA 1-192) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
RAC1 (AA 1-192) protein (GST tag)宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
RAC1 protein (Myc-DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- RAC1
-
背景
-
Abbreviation: Rac1
Target Synonym: Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
Background: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.
-
分子量
-
Calculated MW: 21.1 kDa
Observed MW: 27 kDa
-
UniProt
- P63000
-
途径
- WNT signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
抗原
-
