ITCH Protein (AA 485-862)
This Recombinant ITCH protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for ITCH Protein (AA 485-862) (ABIN7504453)
抗原
See all ITCH 蛋白
ITCH
(Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
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产品特性
- AA 485-862
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原理
- Human ITCH Protein
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序列
- Arg485-Glu862
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产品特性
- Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 197-542) protein (His-IF2DI Tag)
ITCH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) protein (GST tag)ITCH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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别名
- ITCH
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背景
- Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.
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分子量
- 44.98 kDa same as Tris-Bis PAGE result.
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NCBI登录号
- NP_113671
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途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
抗原
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