电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

HLA-E*01:03 (Monomer) protein (Biotin,no Peptide)

宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7504416
发货至: 中国
  • 抗原
    HLA-E*01:03
    蛋白类型
    Recombinant
    产品特性
    Monomer
    宿主
    资源
    • 3
    HEK-293 Cells
    标记
    Biotin,no Peptide
    原理
    Biotinylated Human HLA-E*01:03 & B2M Monomer Protein
    序列
    Gly25-Ile305 (HLA-E*01:03) & Ile21-Met119 (B2M)
    特异性
    Uni-Prot: P13747 (HLA-E*01:03), P61769 (B2M)
    产品特性
    Recombinant Biotinylated Human HLA-E*01:03 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Ile305(HLA-E*01:03) and Ile21-Met119(B2M).
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
  • 应用备注
    Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
    说明

    The HLA-E*01:03&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    HLA-E*01:03
    分子量
    48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
    UniProt
    P13747
You are here:
客服