HLA-E*01:03 (Monomer) protein (Biotin,no Peptide)
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 抗原
- HLA-E*01:03
- 蛋白类型
- Recombinant
- 产品特性
- Monomer
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- Biotin,no Peptide
- 原理
- Biotinylated Human HLA-E*01:03 & B2M Monomer Protein
- 序列
- Gly25-Ile305 (HLA-E*01:03) & Ile21-Met119 (B2M)
- 特异性
- Uni-Prot: P13747 (HLA-E*01:03), P61769 (B2M)
- 产品特性
- Recombinant Biotinylated Human HLA-E*01:03 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Ile305(HLA-E*01:03) and Ile21-Met119(B2M).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
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- 应用备注
- Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
- 说明
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The HLA-E*01:03&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HLA-E*01:03
- 分子量
- 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- P13747
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