MHC, Class I Protein (Monomer) (Ovalbumin)
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 抗原 See all MHC, Class I products
- MHC, Class I
- 蛋白类型
- Recombinant
- 产品特性
- Monomer
- 宿主
- 小鼠
- 资源
- HEK-293 Cells
- 标记
- This MHC, Class I protein is labelled with Ovalbumin.
- 原理
- Mouse H-2K (b) & B2M & OVA (SIINFEKL) Monomer Protein
- 序列
- His24-Pro297, Ile21-Met119 (B2M) and SIINFEKL peptide
- 特异性
- Uni-Prot: P01901 (H-2K (B)), P01887 (B2M), SIINFEKL
- 产品特性
- Recombinant Mouse H-2K(b) & B2M & OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297, Ile21-Met119(B2M) and SIINFEKL peptide.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MHC, Class I
- 别名
- H-2K (MHC, Class I 产品)
- 别名
- H-2K Protein, H-2K(d) Protein, H2-K Protein, K-f Protein, Y-FA Protein, YFV Protein, histocompatibility 2, K1, K region Protein, MHC class I antigen YF5 Protein, MHC class I antigen Protein, major histocompatibility complex, class I Protein, H2-K1 Protein, YF5 Protein, OVAR Protein, LOC512672 Protein
- 背景
- Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
- 分子量
- 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P01901
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