Recombinant Human FSH alpha & beta Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
宿主: 人
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
FSH (Follicle-stimulating hormone) is a gonadotropin, a glycoprotein polypeptide hormone. It has a functionally indispensable 96 amino acid α subunit that is common to LH, TSH and hCG, in addition to a structurally unique β subunit. FSH is synthesized and secreted by the gonadotropic cells of the anterior pituitary gland and regulates the development, growth, pubertal maturation, and reproductive processes of the body.
分子量
10.20 kDa (FSH alpha) and 39.2 kDa (FSH beta). Due to glycosylation, the protein migrates to 25-30 kDa (FSH alpha) and 50-60 kDa (FSH beta) based on Tris-Bis PAGE result.