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- 抗原
- ADGRE5
- 蛋白类型
- Synthetic Nanodisc
- 宿主
- 人
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资源
- HEK-293 Cells
- 原理
- Human ADGRE5 full length protein-synthetic nanodisc
- 产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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ADGRE5 (AA 21-552) protein (His tag)
宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- 说明
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 有效期
- 12 months
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- 抗原
- ADGRE5
- 别名
- TM7LN1 Protein, adhesion G protein-coupled receptor E5 Protein, ADGRE5 Protein
- 背景
- This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
- 分子量
- The human full length ADGRE5 protein has a MW of 87.1 kDa
- UniProt
- P48960
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