SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (Pro13Leu-Mutant) (His tag)
SARS-CoV-2 N
宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 N
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.
分子量
predicted molecular mass of 46.3 kDa after removal of the signal peptide. The apparent molecular mass of Nucleocapsid (D63G, R203M, D377Y)-His is 55-70 kDa due to glycosylation.